Pyrodiversity is the spatial or temporal variability in fire effects across a landscape. Multiple ecological hypotheses, when applied to the context of post-fire systems, suggest that high pyrodiversity will lead to high biodiversity. This resultant “pyrodiversity–biodiversity” hypothesis has grown popular but has received mixed support by recent empirical research. In this paper, we sought to review the existing pyrodiversity literature, appraise support for the pyrodiversity–biodiversity hypothesis, examine potential mechanisms underlying the hypothesis and identify outstanding questions about pyrodiversity and future research needs.