Scenarios have been recognized as a powerful tool for exploring plausible future dynamics and uncertainty in complex systems. Yet, produced scenarios are not used enough, and there is a lack of existing guidance on how to ensure that scenarios are relevant to stakeholders and ultimately properly used in decision-making contexts. This handbook intends to fill this gap for biodiversity scenarios in particular. It was produced in the context of a BiodivERsA-Belmont Forum joint Action (BiodivScen) to support international research on scenarios of biodiversity and ecosystem services. This included funding n multidisciplinary research projects that integrate the scenarios approach into their research, and other activities ranging from capacity building to outreach. One of the objectives of this Action is to promote the science/society – science/policy interfacing within the funded projects and to create capacity for the use of their scenarios as decision-making tools at different scales.