Systematic appraisal of community’s knowledge as evidence for biodiversity conservation has been widely recognized. For conserving the rich biodiversity in the rural landscape outside the protected areas, it is important to document the knowledge and perception of the farming community. Although such appraisal is available for different taxa, no such systematic study is available for herpetofauna- one of the most vulnerable faunal groups.Our study attempts to document the impact of agricultural intensification on herpetofauna in an agricultural landscape through knowledge and perception appraisal of the farming community. A semi-structured questionnaire survey and validation was conducted in areas of low, medium, and high agricultural intensification. In all areas, farmers indicated an overall decrease in herpetofauna abundance. Farmers at the mid and high agricultural intensification zones reported a more significant decrease in herpetofauna sightings specifically for amphibians and snakes compared to those under low intensification regions. Farmers at low intensification area recognized significantly more herpetofauna. Farmers attributed five major threats to herpetofauna and ranked pesticide as the most significant reason, especially those in higher intensification. The majority were aware of the importance of herpetofauna as a biological pest control agent. Level of education or farming experience did not seem to have any influence on the farmers’ knowledge. Our findings integrated with other quantitative studies will facilitate future community-driven conservation in the studied agricultural landscapes.