The 1992 Convention on Biological Diversity (CBD) challenged signatory countries to seek and encourage new financial resources, including private-sector resources, to implement the objectives of the Convention. During the past decade, pioneering businesses have succeeded at making money while conserving and sustainably using biodiversity. What lessons have we learned? How can we speed up the adoption of biodiversity-friendly business activities? Where and how can additional private investment for biodiversity conservation and sustainable use be found? To answer these questions, this article reviews why biodiversity conservation needs private-sector finance. It defines the beneficial links between business and biodiversity; presents the business rationale for financing conservation and sustainable use; identifies who is currently investing in these businesses; and outlines the conditions necessary to attract considerably more amounts of private-sector financing for biodiversity conservation and sustainable use.