Addressing gender inequality in biodiversity conservation is fundamental to meeting the goals and targets of the Convention on Biological Diversity’s (CBD) Post-2020 Global Biodiversity Framework, and building synergies with the Sustainable Development Goals. There are positive outcomes for nature, equity and sustainability, and for overall community wellbeing when women access and control biodiversity and natural resources, can benefit equally from nature, and participate meaningfully in biodiversity-related decision making. This briefing provides evidence of the value of integrating gender into conservation interventions, suggesting that Parties to the CBD should therefore prioritise the gender-responsive implementation of the Post-2020 Global Biodiversity Framework, using the Gender Plan of Action as a guiding mechanism. It identifies key avenues for effective action on the ground, based on evidence from successful interventions.