Trifolium glanduliferum Boiss. var. nervulosum (Boiss. & Heldr.) Zoh., glandular (or gland) clover is an annual pasture species, with a Mediterranean origin, recently introduced into Australia. The species was previously reported as self-pollinating. Studies with four accessions originating from Israel clearly suggest that Trifolium glanduliferum is a highly self-incompatible species, which requires vectors including bees to ensure pollination. Unpollinated bagged and hand-tripped florets showed no signs of pollen tube growth and no seed set, while cross-pollinated florets showed pollen tube growth and gave a high seed set. High pollen: ovule ratio suggested that the species is between facultative outcrossing and obligate outcrossing.